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alpha300r confocal raman microscope  (Oxford Instruments)


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    Structured Review

    Oxford Instruments alpha300r confocal raman microscope
    Alpha300r Confocal Raman Microscope, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 17226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/confocal+microscope/alpha300/pmc13657018-152-7-6
    Average 99 stars, based on 17226 article reviews
    alpha300r confocal raman microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Protocol for detecting protein S-glutathionylation in Arabidopsis thaliana under oxidative stress using a non-transgenic chemical toolkit.
    Article Snippet: .. Confocal Microscope (e.g., Andor Dragonfly): CalFluor 488: Excitation at 488 nm, Emission filter 525 nm. ..

    Article Title: Gate-imprinted memory and light-induced erasure of superconductivity at KTaO 3 -based interfaces.
    Article Snippet: .. SHG measurements A confocal microscope (WITec, Alpha300RAS) equipped with a 1064 nm excitation laser was used for the SHG measurements. ..

    Article Title: Suppressing electron-phonon coupling in perovskite nanoplatelets for efficient pure-red light-emitting diodes with narrow spectral emission.
    Article Snippet: .. Temperature-dependent steady-state PL spectra were recorded using a confocal microscope (Witec Alpha300R) equipped with a liquid-nitrogen-cooled cryostat, with a 405 nm laser as the excitation source. .. Temperature-dependent steady-state PL spectra were recorded using a confocal microscope (Witec Alpha300R) equipped with a liquid-nitrogen-cooled cryostat, with a 405 nm laser as the excitation source.

    Article Title: Digital defocus aberration interference for automated optical microscopy.
    Article Snippet: .. The confocal system was built on a commercial confocal microscope (Andor, Dragonfly 200) with an objective lens (HC PL FLUOTAR 10×/0,30 NA) and a camera (Andor ZL41 Cell sCMOS). ..


    Article Title: Biomimetic bone calcium phosphate-based scaffolds fabricated via ceramic vat photopolymerization: Effect of porosity, sintering temperature, mineralogical phases and trace elements on the osteogenic potential
    Article Snippet: Samples were imaged using Leica DMi8 Inverted Microscope and Zeiss LSM 780 Confocal Microscope (Zeiss Microscopy, Jena, Germany) with 10× air immersion objective at a resolution of 2048 × 2048 pixels and image size of 1062.7 × 1062.7 μm; z-step size of 1.7 μm was used. .. Images taken with the inverted microscope were processed using Fiji ImageJ software (version 2.3.0) and the images taken with the confocal microscope using Imaris Microscopy Image Analysis software (version 9.9.1, Oxford Instruments, Abingdon, UK). ..

    Article Title: Cryopreservable dopaminergic progenitors derived from human iPSCs with accelerated loss of pluripotency and early functional restoration in Parkinsonian rats.
    Article Snippet: The primary antibodies used in this study included FOXA2 (1:100, sc-6554, RRID:AB_2262810) and SOX1 (1:200, sc-17318, RRID:AB_2195365) (Both from Santa Cruz Biotechnology); TH (1:200, AB152, RRID:AB_390204, Sigma-Aldrich), GIRK2 (1:100, ab65096, RRID:AB_1139732), DARPP32 (1:100, ab40801, RRID:AB_731843) and Human Nuclear Antigen (HuNu; 1:200, ab191181, RRID:AB_2885016) (All from Abcam); and STEM121 (1:500, Y40410, RRID:AB_2801314, Takara Bio, Kusatsu, Shiga, Japan). .. Cell nuclei were stained with DAPI, and fluorescence images were captured using a fluorescence AR TI CL E IN P RE SS microscope (BX61VS, Olympus Corporation) and a confocal microscope (ANDOR BC43, Oxford Instruments, Abingdon-on-Thames, Oxford, UK). .. Finally, images were processed using Olympus CellSens Dimension Desktop 3.1 and IMARIS (Oxford Instruments).

    Article Title: The PPTC7/BNIP3/NIX axis induces cGAS/STING-mediated senescence and augments CAR-T efficacy by repressing tumor-intrinsic mitophagy.
    Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (ab150077, 1:1,000, Abcam, UK) was applied for 1 h at room temperature, followed by nuclear counterstaining with DAPI (#4083, Cell Signaling Technology, USA) for 10 min. .. Images were acquired using a confocal microscope (Dragonfly, Andor, Oxford, UK), and colocalization analysis was performed using NIS Elements software (Nikon Precision, China). ..

    Confocal:

    Article Title: Gate-imprinted memory and light-induced erasure of superconductivity at KTaO 3 -based interfaces.
    Article Snippet: .. SHG measurements A confocal microscope (WITec, Alpha300RAS) equipped with a 1064 nm excitation laser was used for the SHG measurements. ..

    Inverted Microscopy:

    Article Title: Biomimetic bone calcium phosphate-based scaffolds fabricated via ceramic vat photopolymerization: Effect of porosity, sintering temperature, mineralogical phases and trace elements on the osteogenic potential
    Article Snippet: Samples were imaged using Leica DMi8 Inverted Microscope and Zeiss LSM 780 Confocal Microscope (Zeiss Microscopy, Jena, Germany) with 10× air immersion objective at a resolution of 2048 × 2048 pixels and image size of 1062.7 × 1062.7 μm; z-step size of 1.7 μm was used. .. Images taken with the inverted microscope were processed using Fiji ImageJ software (version 2.3.0) and the images taken with the confocal microscope using Imaris Microscopy Image Analysis software (version 9.9.1, Oxford Instruments, Abingdon, UK). ..

    Software:

    Article Title: Biomimetic bone calcium phosphate-based scaffolds fabricated via ceramic vat photopolymerization: Effect of porosity, sintering temperature, mineralogical phases and trace elements on the osteogenic potential
    Article Snippet: Samples were imaged using Leica DMi8 Inverted Microscope and Zeiss LSM 780 Confocal Microscope (Zeiss Microscopy, Jena, Germany) with 10× air immersion objective at a resolution of 2048 × 2048 pixels and image size of 1062.7 × 1062.7 μm; z-step size of 1.7 μm was used. .. Images taken with the inverted microscope were processed using Fiji ImageJ software (version 2.3.0) and the images taken with the confocal microscope using Imaris Microscopy Image Analysis software (version 9.9.1, Oxford Instruments, Abingdon, UK). ..

    Article Title: The PPTC7/BNIP3/NIX axis induces cGAS/STING-mediated senescence and augments CAR-T efficacy by repressing tumor-intrinsic mitophagy.
    Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (ab150077, 1:1,000, Abcam, UK) was applied for 1 h at room temperature, followed by nuclear counterstaining with DAPI (#4083, Cell Signaling Technology, USA) for 10 min. .. Images were acquired using a confocal microscope (Dragonfly, Andor, Oxford, UK), and colocalization analysis was performed using NIS Elements software (Nikon Precision, China). ..

    Staining:

    Article Title: Cryopreservable dopaminergic progenitors derived from human iPSCs with accelerated loss of pluripotency and early functional restoration in Parkinsonian rats.
    Article Snippet: The primary antibodies used in this study included FOXA2 (1:100, sc-6554, RRID:AB_2262810) and SOX1 (1:200, sc-17318, RRID:AB_2195365) (Both from Santa Cruz Biotechnology); TH (1:200, AB152, RRID:AB_390204, Sigma-Aldrich), GIRK2 (1:100, ab65096, RRID:AB_1139732), DARPP32 (1:100, ab40801, RRID:AB_731843) and Human Nuclear Antigen (HuNu; 1:200, ab191181, RRID:AB_2885016) (All from Abcam); and STEM121 (1:500, Y40410, RRID:AB_2801314, Takara Bio, Kusatsu, Shiga, Japan). .. Cell nuclei were stained with DAPI, and fluorescence images were captured using a fluorescence AR TI CL E IN P RE SS microscope (BX61VS, Olympus Corporation) and a confocal microscope (ANDOR BC43, Oxford Instruments, Abingdon-on-Thames, Oxford, UK). .. Finally, images were processed using Olympus CellSens Dimension Desktop 3.1 and IMARIS (Oxford Instruments).

    Fluorescence:

    Article Title: Cryopreservable dopaminergic progenitors derived from human iPSCs with accelerated loss of pluripotency and early functional restoration in Parkinsonian rats.
    Article Snippet: The primary antibodies used in this study included FOXA2 (1:100, sc-6554, RRID:AB_2262810) and SOX1 (1:200, sc-17318, RRID:AB_2195365) (Both from Santa Cruz Biotechnology); TH (1:200, AB152, RRID:AB_390204, Sigma-Aldrich), GIRK2 (1:100, ab65096, RRID:AB_1139732), DARPP32 (1:100, ab40801, RRID:AB_731843) and Human Nuclear Antigen (HuNu; 1:200, ab191181, RRID:AB_2885016) (All from Abcam); and STEM121 (1:500, Y40410, RRID:AB_2801314, Takara Bio, Kusatsu, Shiga, Japan). .. Cell nuclei were stained with DAPI, and fluorescence images were captured using a fluorescence AR TI CL E IN P RE SS microscope (BX61VS, Olympus Corporation) and a confocal microscope (ANDOR BC43, Oxford Instruments, Abingdon-on-Thames, Oxford, UK). .. Finally, images were processed using Olympus CellSens Dimension Desktop 3.1 and IMARIS (Oxford Instruments).



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    Nikon nikon c2 confocal microscope
    In vitro properties of Im@NOnanoPL nanovaccine. (A) Flow cytometric analysis of DC2.4 cells co‐incubated with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5 after different durations, respectively. (B) Percentage of Cy5 + DC2.4 cells after incubation with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5. (C) Confocal microscopic images of DC2.4 cells after incubating with Im@NOnanoPL‐Cy5 for 2 h and 6 h. The lysosomes stained with Lysotracker Green (green) and amide‐coupled Cy5 labeling for stable cell membrane vesicles surface modification, Scale bar: 10 µm. (D) Evaluation of MHC‐I levels in BMDCs after 12 and 24 h incubation with Im@NOnanoPL and other formulations (NOC‐18: 100 µM), as detected by flow cytometry ( n = 4). (I: PBS, II: m@nanoPL, III: Im@nanoPL, IV: m@NOnanoPL, V: Im@NOnanoPL). (E) Western blot analysis of cGAS‐STING pathway‐related proteins expression in BMDCs treated by Im@NOnanoPL and other formulations (NOC‐18: 100 µM) in 12 h (G1: PBS, G2: m@nanoPL, G3: Im@nanoPL, G4: m@NOnanoPL, G5: Im@NOnanoPL). (F) Experimental design for T cell activation and cytokine release analysis. BMDCs were treated with different formulations for 12 h, followed by co‐culture with CD3 + T cells at <t>a</t> <t>1:10</t> ratio (BMDCs: T cells). The CD3 + T cells were isolated from splenocytes of MC38 tumor‐bearing mice. (G) Percentages of CD69 + or CD25 + in CD3 + CD8 + T cells after 24 h co‐culture, as detected using flow cytometry ( n = 4). (H) Cytokines released by T cells and DCs from different groups after 24 h co‐culture, including IL‐6, IL‐1β, TNF‐α, IFN‐β. Results are presented as mean ± SD ( n = 4). Statistical analysis was performed using the one‐way ANOVA with Tukey's multiple comparisons test.
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    In vitro properties of Im@NOnanoPL nanovaccine. (A) Flow cytometric analysis of DC2.4 cells co‐incubated with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5 after different durations, respectively. (B) Percentage of Cy5 + DC2.4 cells after incubation with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5. (C) Confocal microscopic images of DC2.4 cells after incubating with Im@NOnanoPL‐Cy5 for 2 h and 6 h. The lysosomes stained with Lysotracker Green (green) and amide‐coupled Cy5 labeling for stable cell membrane vesicles surface modification, Scale bar: 10 µm. (D) Evaluation of MHC‐I levels in BMDCs after 12 and 24 h incubation with Im@NOnanoPL and other formulations (NOC‐18: 100 µM), as detected by flow cytometry ( n = 4). (I: PBS, II: m@nanoPL, III: Im@nanoPL, IV: m@NOnanoPL, V: Im@NOnanoPL). (E) Western blot analysis of cGAS‐STING pathway‐related proteins expression in BMDCs treated by Im@NOnanoPL and other formulations (NOC‐18: 100 µM) in 12 h (G1: PBS, G2: m@nanoPL, G3: Im@nanoPL, G4: m@NOnanoPL, G5: Im@NOnanoPL). (F) Experimental design for T cell activation and cytokine release analysis. BMDCs were treated with different formulations for 12 h, followed by co‐culture with CD3 + T cells at a 1:10 ratio (BMDCs: T cells). The CD3 + T cells were isolated from splenocytes of MC38 tumor‐bearing mice. (G) Percentages of CD69 + or CD25 + in CD3 + CD8 + T cells after 24 h co‐culture, as detected using flow cytometry ( n = 4). (H) Cytokines released by T cells and DCs from different groups after 24 h co‐culture, including IL‐6, IL‐1β, TNF‐α, IFN‐β. Results are presented as mean ± SD ( n = 4). Statistical analysis was performed using the one‐way ANOVA with Tukey's multiple comparisons test.

    Journal: Exploration

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity

    doi: 10.1002/exp2.70238

    Figure Lengend Snippet: In vitro properties of Im@NOnanoPL nanovaccine. (A) Flow cytometric analysis of DC2.4 cells co‐incubated with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5 after different durations, respectively. (B) Percentage of Cy5 + DC2.4 cells after incubation with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5. (C) Confocal microscopic images of DC2.4 cells after incubating with Im@NOnanoPL‐Cy5 for 2 h and 6 h. The lysosomes stained with Lysotracker Green (green) and amide‐coupled Cy5 labeling for stable cell membrane vesicles surface modification, Scale bar: 10 µm. (D) Evaluation of MHC‐I levels in BMDCs after 12 and 24 h incubation with Im@NOnanoPL and other formulations (NOC‐18: 100 µM), as detected by flow cytometry ( n = 4). (I: PBS, II: m@nanoPL, III: Im@nanoPL, IV: m@NOnanoPL, V: Im@NOnanoPL). (E) Western blot analysis of cGAS‐STING pathway‐related proteins expression in BMDCs treated by Im@NOnanoPL and other formulations (NOC‐18: 100 µM) in 12 h (G1: PBS, G2: m@nanoPL, G3: Im@nanoPL, G4: m@NOnanoPL, G5: Im@NOnanoPL). (F) Experimental design for T cell activation and cytokine release analysis. BMDCs were treated with different formulations for 12 h, followed by co‐culture with CD3 + T cells at a 1:10 ratio (BMDCs: T cells). The CD3 + T cells were isolated from splenocytes of MC38 tumor‐bearing mice. (G) Percentages of CD69 + or CD25 + in CD3 + CD8 + T cells after 24 h co‐culture, as detected using flow cytometry ( n = 4). (H) Cytokines released by T cells and DCs from different groups after 24 h co‐culture, including IL‐6, IL‐1β, TNF‐α, IFN‐β. Results are presented as mean ± SD ( n = 4). Statistical analysis was performed using the one‐way ANOVA with Tukey's multiple comparisons test.

    Article Snippet: Imaging was performed on a Nikon A1R HD25 confocal microscope using a 60× oil objective.

    Techniques: In Vitro, Analysis, Incubation, Confocal, Staining, Labeling, Stable Transfection, Membrane, Modification, Flow Cytometry, Western Blot, Expressing, Activation Assay, Co-culture Assay, Isolation